Basic Violet 14 CAS 632-99-5
1. Versatile Applications: Basic fuchsin is widely used in biological, plant, and bacteriological studies, including DNA verification and tuberculosis identification.
2. Effective Staining: It effectively stains collagen, elastic fibers, and various tissues, making it a valuable tool in histology and microbiology.
3. Solubility: Basic fuchsin is soluble in both water and alcohol, providing flexibility for different staining preparations.
4. Stable When Stored Properly: It maintains its staining properties when stored away from light and at the right pH levels.
This refers to aniline dyes or coal tar dyes, which come in numerous varieties and have extensive applications. Their primary drawback is their tendency to fade when exposed to sunlight, with colors like aniline blue, brilliant green, and methyl green being particularly susceptible to fading. When preparing solutions, attention should be paid to maintaining proper pH levels and avoiding direct sunlight exposure; with proper care, these dyes can maintain their color for several years without significant fading.
Basic fuchsin (also known as Basic fuchsin in English) is an alkaline dye appearing as a dark red powder or crystalline substance. It demonstrates solubility in both water (with a solubility of 1%) and alcohol (with a solubility of 8%). This dye finds wide application in biological preparations, where it's employed to stain collagen fibers, elastic fibers, fuchsin granules, and nuclei in central nervous tissue. In plant biology preparations, it serves to color the lignified walls of vascular plants and functions as a comprehensive stain for protozoa and charophytes.
In bacteriological applications, basic fuchsin is commonly used for tuberculosis bacteria identification. It also serves as a histochemical reagent in the Feulgen reaction and has been utilized for deoxyribonucleic acid (DNA) verification. The dye maintains its staining properties effectively when stored properly away from light and at appropriate pH conditions.
Melting point | 250 °C |
Boiling point | 300℃[at 101 325 Pa] |
density | 0.999 g/mL at 20 °C |
bulk density | 500kg/m3 |
vapor pressure | 0Pa at 25℃ |
refractive index | n20/D 1.334 |
Fp | 48 °F |
storage temp. | 2-8°C |
solubility | H2O: soluble1mg/mL |
form | liquid |
Colour Index | 42510 |
color | Green to dark green |
PH | 5-6 (1g/l, H2O, 25°C) |
Water Solubility | 4 G/L (25 ºC) |
λmax | 543 nm |
Merck | 14,5652 |
BRN | 4166684 |
Biological Applications | Detecting cancer; treating Jock itch,oral fungal infection,piles; periodontal disease |
LogP | 1.632 at 25℃ |
IARC | 2B (Vol. Sup 7, 57, 99, 100F) 2012 |
EPA Substance Registry System | C.I. Basic Violet 14 (632-99-5) |
Safety Information
Hazard Codes | F,Xn |
Risk Statements | 40-22 |
Safety Statements | 7-16-36/37 |
WGK Germany | 3 |
RTECS | CX9850000 |
TSCA | Yes |
HS Code | 3204 13 00 |
Hazardous Substances Data | 632-99-5(Hazardous Substances Data) |




